Characterisation of Commensal Escherichia coli Isolated from Apparently Healthy Cattle and Their Attendants in Tanzania

نویسندگان

  • Balichene P. Madoshi
  • Egle Kudirkiene
  • Madundo M. A. Mtambo
  • Amandus P. Muhairwa
  • Athumani M. Lupindu
  • John E. Olsen
چکیده

While pathogenic types of Escherichia coli are well characterized, relatively little is known about the commensal E. coli flora. In the current study, antimicrobial resistance in commensal E. coli and distribution of ERIC-PCR genotypes among isolates of such bacteria from cattle and cattle attendants on cattle farms in Tanzania were investigated. Seventeen E. coli genomes representing different ERIC-PCR types of commensal E. coli were sequenced in order to determine their possible importance as a reservoir for both antimicrobial resistance genes and virulence factors. Both human and cattle isolates were highly resistant to tetracycline (40.8% and 33.1%), sulphamethazole-trimethoprim (49.0% and 8.8%) and ampicillin (44.9% and 21.3%). However, higher proportion of resistant E. coli and higher frequency of resistance to more than two antimicrobials was found in isolates from cattle attendants than isolates from cattle. Sixteen out of 66 ERIC-PCR genotypes were shared between the two hosts, and among these ones, seven types contained isolates from cattle and cattle attendants from the same farm, suggesting transfer of strains between hosts. Genome-wide analysis showed that the majority of the sequenced cattle isolates were assigned to phylogroups B1, while human isolates represented phylogroups A, C, D and E. In general, in silico resistome and virulence factor identification did not reveal differences between hosts or phylogroups, except for lpfA and iss found to be cattle and B1 phylogroup specific. The most frequent plasmids replicon genes found in strains from both hosts were of IncF type, which are commonly associated with carriage of antimicrobial and virulence genes. Commensal E. coli from cattle and attendants were found to share same genotypes and to carry antimicrobial resistance and virulence genes associated with both intra and extraintestinal E. coli pathotypes.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Prevalence and molecular characterization of verotoxin-producing Escherichia coli O157:H7 in cattle and sheep in Shiraz-Iran

Shiga toxin producing Escherichia coli have been associated with HUS, HC and TTP in human. We found recto-anal mucosal sample in sheep as well in cattle is the main site for E. coli O157 localization. 1246 E. coli isolates from 872 both healthy and diarrheic animals were analyzed, by screening for the presence of Shiga toxin-producing (VT 1 and VT 2) and intimin (eae) genes used Multiplex PCR. ...

متن کامل

A study on the presence of some potential virulence genes and quinolone resistance in avian pathogenic Escherichia coli isolated from chickens in Northeast of Iran

Background: Avian pathogenic Escherichia coli (APEC), which is the causative agent of colibacillosis, harbors several putative virulence genes. An important trait of APEC for both poultry and public health is antibiotic resistance. Objectives: In the present study, some potential virulence genes of APECs isolated from Northeast of Iran and their resistance to the quinolones antibiotics were stu...

متن کامل

تعیین گروه‌های فیلوژنتیک و مقاومت آنتی‌بیوتیکی در ایزوله‌های اشریشیاکلی اوروپاتوژنیک و کامنسال جدا شده از شهر زنجان

Background and Objective: Escherichia coli strains are the major cause of urinary tract infections which belong to the large group of extra-intestinal pathogenic E. coli. They fall into four main phylogenetic groups: A, B1, B2 and D. The aim of the present study was to determine phylogenetic groups in uropathogenic and commensal Escherichia coli isolated. Also, due to increase in the rate of an...

متن کامل

Detection of Colicin genes by PCR in Escherichia coli isolated from cattle in Shiraz-Iran

A variety of probiotic bacteria have been tested to control animal and foodborne pathogenic bacteria in livestock. The mechanism of inhibition of pathogenic bacteria for several of those probiotic microorganisms is mediated by the production of bacteriocins. Colicins are probably the group of bacteriocins that have been most thoroughly characterized. Colicins are antimicrobial proteins produced...

متن کامل

Hyperimmune lipopolysaccharide antiserum mediated inhibition of the adherence of E. coli O157:H7 to HEP-2 cells and large intestine of mice

Escherichia coli O157:H7 is found in cattle farms and can live in the intestine of healthy cattle. Mostcases of human illnesses including nonbloody diarrhea, hemorrhagic colitis and hemolytic uremic syndromecan be traced, either directly or indirectly, to cattle. One strategy for reducing the risk of EnterohemorrhagicEscherichia coli (EHEC) infections in human is to reduce the prevalence of inf...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره 11  شماره 

صفحات  -

تاریخ انتشار 2016